Ontario Highway 28 is a provincial highway in Ontario, Canada. It runs from southwest to northeast and is 162.6 kilometers (101 miles) long. It starts at an intersection with Highway 7 about 7 kilometers (4.3 miles) east of Peterborough. It ends at the town of Denbigh at a junction with Highway 41. The highway originally continued south of Peterborough and into Northumberland County but in 1997 and 1998, the southern 63 kilometers (39 miles) of the highway was transferred to local control.