English: This is a workflow that demonstrates the general strategy in a MeDIP-seq experiment. Particularly, the example investigates cancer as a general source of disease condition; however, the starting source can range from different tissue types or different time points. This diagram outlines, very briefly, the steps in MeDIP-seq including the use of software for aligning short reads depicted at the "High-throughput Sequencing" step. At the anlysis stage, a graph can be used to visualize and compare methylation levels between experimental sample (i.e. cancer/disease state, tissue-specific, time-specific, etc.) and the control (i.e. normal/blood cells, baseline time-point, normal tissue, etc.). The peaks show in the plot can essentially represent the frequency or short reads that have been aligned to the particular region in the genome. The more reads that cover a particular region, then the higher the methylation level. This can be explained by MeDIP having enriched for methylated DNA at this region. The plot and data in this diagram is entirely simulated and does not represent real MeDIP-seq data; this is to simply display typical visualization that can be used during MeDIP-seq analysis.
This is a retouched picture, which means that it has been digitally altered from its original version. Modifications: svg. The original can be viewed here: Study-flow.jpg: .
Licensing
I, the copyright holder of this work, hereby publish it under the following license:
Public domainPublic domainfalsefalse
I, the copyright holder of this work, release this work into the public domain. This applies worldwide. In some countries this may not be legally possible; if so: I grant anyone the right to use this work for any purpose, without any conditions, unless such conditions are required by law.
Original upload log
This image is a derivative work of the following images:
2009-03-03T06:07:39Z Gavinha 984x1508 (127960 Bytes) {{Information |Description={{en|1=This is a workflow that demonstrates the general strategy in a MeDIP-seq experiment. Particularly, the example investigates cancer as a general source of disease condition; however, the star
== {{int:filedesc}} == {{Information |Description={{en|1=This is a workflow that demonstrates the general strategy in a MeDIP-seq experiment. Particularly, the example investigates cancer as a general source of disease condition; however, the starting...