Talk:Nuclear run-on

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I cannot find the original developers! people tell me that it is a pre PCR technique but I cannot find anythinbg older than 1981 on pubmed.. —Preceding unsigned comment added by Squidonius (talk • contribs) 16:32, 31 August 2007 (UTC)[reply]

The thing to do back then was to fractionate your cellular parts or your mangled nuclei, so a lot of old papers discuss the a-amanitin sensitive or resistant properties of their nuclear fractions -- in order to determine which fraction contained which RNA polymerase. Check out Roeder & Rutter's work over the late 60s/early 70s and decide for yourself which protocols fall into the "nuclear run-on" category vs. the "biochemistry without an FPLC" category. Here's one: http://www.pnas.org/content/65/3/675 21:31, 2 May 2009 (UTC)J. Sims —Preceding unsigned comment added by 140.247.244.44 (talk)